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I staterted out with 2 - one for my buisness and one personal but I have stopped posting to my business one.
WHY?
Because people respond to people not businesses. I am currnetly studying a social media course under the biggest expert in Social Media (this guy started from scratch and made over $600,000 in just 9 months on Twitter so he knows his stuff!)
His advice is just have the one Twitter account. It works best because twitter is unlike any other social media - people are on there for diversity. (a load of other reasons too but too numerous to go into and also don't want to give away his whole system!)
Just some food for thought
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